amplificatietesten
Amplificatietesten are diagnostic tests that detect biological targets by amplifying nucleic acids. The principle is to increase the quantity of a specific DNA or RNA sequence so that it can be detected even when present in very small amounts. The most common approaches are nucleic acid amplification tests (NAATs), including polymerase chain reaction (PCR)-based methods and isothermal amplification techniques.
PCR-based methods include conventional PCR, real-time quantitative PCR (qPCR), reverse transcription PCR (RT-PCR) for RNA targets,
Applications span medical diagnostics, infectious disease surveillance, genetic and oncology testing, and environmental or food safety
A typical workflow includes specimen collection, extraction of nucleic acids, the amplification step, and detection of
Advantages of amplification tests include high sensitivity, specificity, and the possibility of multiplexing. Limitations include the