U153C

U153C denotes a single-nucleotide variant in an RNA sequence in which the nucleotide uracil (U) at position 153 is replaced by cytosine (C). The notation assumes a reference sequence and standard 5' to 3' numbering. U153C can be reported for various RNA contexts, including viral genomes, ribosomal or transfer RNA, messenger RNA, or other RNA transcripts.

Numbering schemes vary by reference sequence and genome organization; the same label could refer to different positions in different organisms or genes. Researchers typically specify the reference, gene name, and whether the position is relative to the genome, transcript, or coding sequence.

Functional consequences depend on context. In coding regions, the substitution may be synonymous (no amino acid change) or nonsynonymous (altering an amino acid); in noncoding regions, it may affect RNA folding, regulatory motifs, splicing signals, or interactions with proteins and ligands. The impact is assessed experimentally or via computational predictions.

Detection methods include sequencing approaches such as next-generation sequencing or Sanger sequencing; confirmation may involve replicates and independent assays. In studies, U153C is analyzed alongside other variants to track evolution, assess phenotype, or study structure–function relationships in the RNA.

Because multiple reference sequences exist, and numbering can differ, the significance of U153C must be interpreted within its specific genomic and experimental context. This article provides a general definition rather than a characterization of a particular U153C mutation, which would require explicit reference to a gene and study.