Ser616

Ser616 is a designation used in protein science to indicate a serine residue at position 616 within a specific protein sequence. The meaning of Ser616 is not fixed to a single protein; rather, the same residue number can refer to different amino acids in different proteins, species, or isoforms, depending on how the protein’s sequence is annotated.

In many cases, Ser616 is discussed as a potential site of post-translational modification, most commonly phosphorylation. Phosphorylation of serine residues can influence a protein’s enzymatic activity, interactions with other molecules, subcellular localization, or stability. The functional impact of Ser616 is therefore context-dependent and must be interpreted in relation to the particular protein and cellular pathway involved.

Methods for studying Ser616 include mass spectrometry-based proteomics to identify and quantify phosphorylation at that site, and site-directed mutagenesis (for example, substituting serine with alanine to prevent phosphorylation or with aspartate/glutamate to mimic a phosphorylated state) to assess functional consequences. Phospho-specific antibodies can also be developed for targeted detection in specific proteins.

numbering and annotation: Ser616 highlights illustrate how residue numbering is tied to a reference protein sequence and isoforms. Differences in isoform length or species can shift residue positions, so accurate interpretation requires specifying the exact protein, its accession, and the organism.

See also: protein phosphorylation, serine phosphorylation, post-translational modification, protein sequence numbering.