Seq21215

Seq21215 is a reference accession used in genomics education and example datasets to demonstrate how sequence records are organized and annotated. It does not correspond to a verified natural sequence in any organism; instead it is presented as a synthetic construct intended for teaching and software testing.

The record describes a 512-nucleotide synthetic DNA sequence with no known biological host. The nucleotide composition is balanced, with a GC content around 54 percent. The annotation section includes a predicted coding sequence spanning nucleotides 60 to 300, which would encode a hypothetical protein of about 74 amino acids if translated. This annotation is computational and lacks experimental validation, and no functional domains are asserted.

The entry provides standard metadata fields such as organism designation listed as synthetic construct, isolation source as educational dataset, and a generation date corresponding to its inclusion in a teaching collection. Because Seq21215 is synthetic, its sequence and features are not intended to represent real biology and should be used solely for instructional purposes, algorithm testing, or primer-design practice.

In practice, Seq21215 can be used to illustrate steps of sequence retrieval, feature annotation, ORF finding, and comparative analysis in bioinformatics workflows. Researchers should treat it as a non-authentic sequence and refer to real accession numbers for empirical studies.