RNAalong
RNAalong is an emerging concept in transcriptomics that describes a method to map regulatory features along the entire length of RNA molecules in a single assay. The approach aims to integrate information about RNA–protein interactions, RNA modifications, and secondary structure across full-length transcripts by combining crosslinking-based capture with long-read sequencing.
Conceptually, RNAalong begins with stabilization of RNA–protein contacts by UV or chemical crosslinking, followed by preparation
The resulting data can yield base-resolution maps of occupancy by RNA-binding proteins, regions of structured RNA,
Applications include investigations of post-transcriptional regulation, viral RNA biology, and complex eukaryotic transcriptomes. Compared with fragmentary
Status and outlook: as an emerging concept, RNAalong remains under development, with ongoing work to optimize