RNAaffiniteitschapering
RNA affinity refers to the strength of interaction between an RNA molecule and a binding partner, such as a protein, another RNA, or a small molecule. Affinity is commonly quantified by the equilibrium dissociation constant (Kd); lower values indicate tighter binding. Relative affinities span from high (low nanomolar to picomolar Kd) to moderate and low (micromolar to millimolar). The kinetics of binding are described by kon and koff, with Kd = koff / kon.
RNA affinity is influenced by sequence and structure. Primary sequence dictates binding motifs, while secondary and
Measurement approaches include electrophoretic mobility shift assays (EMSAs), filter binding assays, surface plasmon resonance (SPR), bio-layer