RNAVelocity
RNAVelocity is a computational framework and software package designed to estimate RNA velocity from single-cell RNA sequencing data. It provides a unified approach to infer the directional dynamics of gene expression by analyzing the balance between spliced and unspliced transcripts, enabling the reconstruction of cellular trajectories and lineage relationships in complex tissues.
The package implements both deterministic and probabilistic models of transcriptional dynamics and is designed to work
Key features include data preprocessing tools for quality control, normalization, and gene filtering; versatile velocity estimation
Typical workflows start with preparing spliced and unspliced transcript counts, followed by running RNAVelocity to compute
Limitations include reliance on the assumption that spliced/unspliced ratios reflect transcriptional dynamics and sensitivity to data
See also: RNA velocity, velocyto, scVelo, single-cell transcriptomics.