PhosphoGRID

PhosphoGRID is a publicly accessible database that curates experimentally validated phosphorylation sites and kinase–substrate relationships, with a focus on Saccharomyces cerevisiae. It collects high-confidence in vivo phosphorylation events reported in the scientific literature and maps them to specific residues on target proteins. Each entry typically includes the phosphorylated residue and position, the protein involved, the experimental evidence supporting the modification, the cellular context or condition under which the site was observed, and the reference to the published study. When known, the responsible kinase or kinase complex is noted, enabling users to trace signaling relationships within the yeast phosphoproteome.

The data in PhosphoGRID are curated from peer-reviewed publications and are designed to support systematic analyses of phosphorylation networks. The database emphasizes site-level detail, allowing researchers to examine how individual phosphorylation events contribute to protein function, signaling pathways, and cellular responses. Contextual information, such as experimental method and conditions, helps distinguish robust phosphorylation events from potential artefacts and facilitates cross-study comparisons.

Access and use: PhosphoGRID provides a web interface for searching and filtering entries by protein, residue, kinase, or publication. Entries are cross-referenced with related resources, notably the Saccharomyces Genome Database and other proteomics resources, to support integrative studies. The downloadable data and updates enable researchers to incorporate phosphorylation information into computational models, phosphoproteomic analyses, and network reconstructions. PhosphoGRID serves as a key resource for studies of yeast signaling and phosphoregulation.