Nucleofilt
Nucleofilt is a category of filtration-based methods used to purify nucleic acids from complex biological samples. The approach combines membrane-based size exclusion with charge interactions to retain DNA or RNA while allowing proteins, salts, and inhibitors to be washed away. It is not a single patented device but a family of disc-, cartridge-, and inline filtration formats used to prepare nucleic acids for downstream applications. Nucleofilt methods aim to be rapid, gentle on nucleic acids, and compatible with a range of sample types.
Operation typically involves applying a sample to a filtration device, using centrifugal or vacuum-driven flow to
Applications include isolation of genomic DNA, plasmid DNA, and total RNA from tissues or cells, as well
Limitations and considerations include binding capacity limits, potential loss of very large DNA fragments, and the
Origin and usage: the term nucleofilt describes a class of filtration-based purification approaches rather than a