Multiplexkleuring

Multiplexkleuring is a technique used in histology and molecular biology to simultaneously visualize multiple different targets within a single tissue sample or cell. This allows researchers to study the spatial relationships and co-localization of different molecules, such as proteins or nucleic acids, in a highly efficient manner. The core principle involves using different fluorescent dyes or labels, each with a unique excitation and emission spectrum, to bind to specific targets.

The process typically begins with preparing the tissue or cell sample. This may involve fixation, embedding, and sectioning. Then, a cocktail of antibodies or probes, each conjugated to a distinct fluorophore, is applied to the sample. These antibodies or probes are designed to bind specifically to the targets of interest. After incubation and washing to remove unbound reagents, the sample is viewed under a fluorescence microscope equipped with appropriate filters to excite and detect the emitted light from each fluorophore. By capturing images at different wavelengths or using spectral unmixing techniques, researchers can differentiate and map the locations of multiple targets. Multiplexkleuring significantly reduces the time and resources required compared to performing multiple single-labeling experiments, making it a valuable tool for accelerating research in fields like cancer biology, neuroscience, and immunology.