Kiertokromatografiassa

Kiertokromatografia, often referred to as circular chromatography or simply "kierto," is a chromatographic technique where the mobile phase flows in a circular path. This differs from conventional linear chromatography where the mobile phase moves from one end of the stationary phase to the other. In kiertokromatografia, the stationary phase is typically packed into a disk or a ring, and the mobile phase is introduced at the center and allowed to migrate radially outwards or inwards, depending on the specific design. As the mobile phase moves through the stationary phase, components of the sample separate based on their differential interactions with both the stationary and mobile phases.

The circular nature of the flow can offer several advantages. It can allow for longer separation paths within a smaller footprint compared to linear columns, potentially leading to improved resolution. Furthermore, some kiertokromatografia systems are designed for continuous or semi-continuous operation, making them suitable for applications requiring ongoing sample processing. The development of kiertokromatografia has explored various configurations and applications, including analytical separations and preparative chromatography for purifying larger quantities of substances. The principle of separation remains similar to other chromatographic methods, relying on partitioning, adsorption, or ion exchange mechanisms, but the geometry of the flow path is the defining characteristic of this technique.