H460

H460 is a human non‑small cell lung carcinoma cell line that was established from a pleural effusion of a 45‑year‑old male patient with adenocarcinoma of the lung. The cell line was first described in the early 1980s and has since been widely used as a model for lung cancer research. H460 cells are cultured in RPMI‑1640 medium supplemented with 10 % fetal bovine serum and antibiotics. They exhibit an adherent growth pattern and have a population doubling time of approximately 24–30 hours. Morphologically, H460 cells are elongated and display a spindle‑shaped appearance typical of epithelial cancer cells.

Genetically, H460 is characterized by amplification of the MYC oncogene and overexpression of the epidermal growth factor receptor (EGFR). Additionally, the cells carry a K-RAS mutation (G12C), which contributes to their oncogenic phenotype. The presence of these genetic alterations makes H460 a valuable tool for studying targeted therapies against EGFR and RAS pathways.

H460 cells have been used extensively in studies of tumor invasion, metastasis, and drug resistance. They form invasive colonies in vitro and are capable of metastasizing to the lungs and liver in xenograft models. Researchers also use H460 to evaluate the efficacy of chemotherapeutic agents, immunotherapies, and nanoparticles. The cell line’s high rate of proliferation and its well‑documented genetic profile facilitate the investigation of novel therapeutic strategies for advanced lung cancer.