Diafilmes

Diafiltration, often abbreviated as diafiltration, is a process used in biotechnology and pharmaceutical industries to purify proteins and other biomolecules. The term "diafiltration" is derived from the Greek words "dia," meaning "through," and "filtration," referring to the process of filtering. This technique involves the use of a semipermeable membrane to selectively remove or concentrate solutes from a solution while allowing water to pass through.

The process typically begins with a solution containing the biomolecule of interest. This solution is then passed through a membrane with specific pore sizes that allow water molecules to pass through while retaining the biomolecule. By continuously adding fresh solvent to the feed side of the membrane, the concentration of the biomolecule can be adjusted. This process can be repeated multiple times to achieve the desired level of purity and concentration.

Diafiltration is particularly useful for large-scale purification of proteins and other biomolecules due to its high throughput and relatively low cost. It is often used in conjunction with other purification techniques, such as chromatography, to achieve the desired purity and yield. The process can be customized to suit the specific requirements of the biomolecule being purified, making it a versatile tool in the biopharmaceutical industry.

In summary, diafiltration is a filtration process that uses a semipermeable membrane to selectively remove or concentrate solutes from a solution. It is widely used in the purification of proteins and other biomolecules in the biotechnology and pharmaceutical industries.