DNasemediated
DNase-mediated refers to processes and activities that are driven or facilitated by deoxyribonuclease enzymes, which hydrolyze DNA by cleaving phosphodiester bonds. The term typically encompasses actions carried out by DNase I and DNase II, two major families with distinct properties and cellular locations.
DNases are endonucleases that digest DNA into smaller fragments. DNase I is a calcium- and magnesium-dependent
Applications and examples include: removing contaminating DNA from RNA samples in molecular biology workflows; performing DNase
Limitations and safety considerations involve potential off-target DNA degradation, the need for appropriate cofactors and conditions,
See also: DNase footprinting, dornase alfa, nuclease, extracellular DNA.