DEPCbehandlet
DEPCbehandlet refers to liquids or surfaces that have been treated with diethyl pyrocarbonate (DEPC) to inactivate ribonucleases (RNases) and thereby create RNase-free conditions for work involving RNA. The term is commonly used in molecular biology in Danish-speaking contexts to describe water, buffers, glassware, or other materials that have undergone DEPC treatment to reduce RNase activity.
The standard purpose of DEPC treatment is to minimize RNase contamination during RNA isolation, cDNA synthesis,
Safety and handling considerations are important. DEPC is hazardous and should be handled in a fume hood
Limitations include the need for strict handling to prevent recontamination, potential incompatibilities with certain reagents, and