CITEseq
CITE-seq, short for Cellular Indexing of Transcriptomes and Epitopes by Sequencing, is a single-cell multi-omics method that measures both gene expression and cell-surface protein levels in the same cell. The approach combines antibodies conjugated to DNA oligonucleotide tags with standard single-cell RNA sequencing workflows, enabling parallel profiling of two molecular modalities.
In brief, cells are labeled with antibody-oligo conjugates targeting surface proteins of interest. Each antibody carries
CITE-seq is widely used for immune profiling and precise cell type identification, enabling integrated analysis of
Limitations include dependence on high-quality antibodies, potential non-specific binding, and additional cost and complexity. Antibody panels