BCAmääritys
BCA-määritys, also known as the Bicinchoninic Acid Assay, is a biochemical assay used to determine the total protein concentration in a solution. It is a colorimetric assay, meaning it relies on a color change that can be measured spectrophotometrically. The assay works by using bicinchoninic acid to detect cuprous ions (Cu+), which are produced when the protein is reduced by the sample in an alkaline environment.
The BCA-määritys is a two-step process. First, copper ions are reduced by proteins in an alkaline solution,
This assay is known for its sensitivity and tolerance to detergents and other common laboratory reagents, making