totalRNA
totalRNA refers to the complete set of ribonucleic acids extracted from a biological sample, encompassing messenger RNA, ribosomal RNA, transfer RNA, and various non‑coding RNA species. This contrasts with isolated mRNA, which is enriched and often purified by methods such as poly(A) selection or rRNA depletion. By retaining all RNA classes, totalRNA provides a comprehensive snapshot of cellular transcriptional activity and RNA content, which is valuable for studies that require analysis of non‑coding RNAs, small RNAs, or rRNA dynamics.
Extraction of totalRNA typically employs guanidinium thiocyanate-phenol-chloroform protocols or silica‑based column kits. These techniques lyse cells,
Applications of totalRNA include global RNA‑sequencing (RNA‑seq) workflows, small RNA profiling, ribosome profiling, and quantitative PCR