spFRET
spFRET, or single-pair Förster resonance energy transfer, is a powerful technique used in biochemistry and molecular biology to study the dynamics and interactions of biomolecules at the nanoscale. It is based on the principle of Förster resonance energy transfer (FRET), where energy is transferred non-radiatively from a donor fluorophore to an acceptor fluorophore when they are in close proximity.
In spFRET, a single pair of donor and acceptor fluorophores is attached to a biomolecule of interest,
spFRET has several advantages over other FRET-based techniques. It allows for the study of single molecules,
However, spFRET also has its limitations. The technique requires the use of specialized equipment, such as confocal
In summary, spFRET is a valuable tool for studying the dynamics and interactions of biomolecules at the