nonubiquitinated
Nonubiquitinated refers to proteins that have not had ubiquitin covalently attached to them. Ubiquitination is a post-translational modification in which ubiquitin is attached to lysine residues on target proteins through a cascade of enzymes (E1 activating enzymes, E2 conjugating enzymes, and E3 ligases). Ubiquitination can mark proteins for degradation, alter their activity, or change their cellular location, depending on the type of ubiquitin linkage and the number of ubiquitin units attached.
Most attention in ubiquitin biology focuses on polyubiquitination that targets proteins for proteasomal degradation, typically via
Nonubiquitinated status can arise from the absence or inactivity of E3 ligases, the action of deubiquitinases
In research, distinguishing nonubiquitinated from ubiquitinated forms helps illuminate regulatory mechanisms and protein lifetimes. Detection methods