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lactaseproducing

Lactase-producing describes organisms, enzymes, or biotechnological systems that synthesize lactase, the enzyme that hydrolyzes lactose into glucose and galactose. In humans, lactase is produced by enterocytes in the small intestine as lactase-phlorizin hydrolase, with activity regulated by genetic and developmental factors. In the gut microbiome, several microorganisms express lactase as part of their carbohydrate metabolism, contributing to lactose digestion in individuals who consume dairy.

Industrial production of lactase is dominated by microbial sources. The yeasts Kluyveromyces lactis and some strains

Applications include production of lactose-free or reduced-lactose dairy products by hydrolyzing lactose in milk and whey;

Genetic variation in humans influences lactase persistence into adulthood; individuals with lactase non-persistence typically experience reduced

of
Candida,
and
the
filamentous
fungus
Aspergillus
oryzae,
are
commonly
used
to
synthesize
lactase
at
industrial
scale.
Recombinant
methods
in
bacteria
or
yeast
can
increase
yield
and
allow
for
secretion
of
the
enzyme.
The
crude
or
purified
enzyme
is
formulated
as
a
liquid,
powder,
or
immobilized
preparation
for
processing.
improvement
of
sweetness
and
fermentation
properties
in
dairy
products;
and
use
in
infant
formula
and
nutrition
products.
Lactase
is
also
used
in
diagnostic
tests
for
lactose
intolerance
and
in
enzymatic
assays.
lactase
activity
after
weaning.
Enzyme
supplements
containing
lactase
may
help
some
people
digest
dairy
products.
Considerations
for
use
include
dosage,
temperature
and
pH
stability,
and
regulatory
approval.