immunoturbidimetric

Immunoturbidimetric is a laboratory technique used to measure the concentration of specific substances, such as proteins or other biomolecules, in a sample through the assessment of turbidity changes caused by antigen-antibody interactions. This method is widely employed in clinical diagnostics to quantify markers such as immunoglobulins, complement proteins, hormones, and enzymes.

The principle of immunoturbidimetry involves mixing a patient sample with specific antibodies that bind to the target analyte. When the antibody binds to its antigen, it forms immune complexes that cause an increase in the sample's turbidity. The degree of turbidity is measured spectrophotometrically, typically at a wavelength around 340-405 nm, with the increase in absorbance proportional to the concentration of the analyte in the sample. Calibration curves generated using known standards enable precise quantification.

Immunoturbidimetric assays are valued for their speed, automation capability, and high throughput, making them suitable for routine clinical testing. They offer advantages over other immunoassay techniques such as radioimmunoassay or enzyme-linked immunosorbent assay (ELISA), including reduced assay time, minimal sample preparation, and safety considerations due to the absence of radioisotopes.

However, immunoturbidimetry can be affected by interfering substances, lipemia, hemolysis, or high concentrations of proteins, which may cause inaccuracies. Proper sample handling and calibration are essential for reliable results.

Overall, immunoturbidimetric assays are a critical component in modern clinical laboratories, providing efficient and accurate measurements for disease diagnosis, monitoring, and research.