coimmunopresipiering
Coimmunopresipiering, or co-immunoprecipitation (co-IP), is a biochemical method used to test whether two or more proteins interact physically within a cell or tissue. The technique centers on an antibody that specifically recognizes a target protein, called the bait. When a cell or tissue lysate is incubated with the antibody and captured on beads, proteins bound to the bait, the prey, are co-precipitated and enriched for analysis.
The basic workflow involves preparing a non-denaturing lysate to preserve protein interactions, incubating it with an
Interpretation of results requires appropriate controls. A successful co-IP shows enrichment of a prey protein in
Limitations and caveats include dependence on antibody quality and epitope accessibility, choice of detergent and lysis