chromatographybased
Chromatographybased describes methods and processes that rely on chromatography as the primary mechanism to separate components of a mixture. In chromatography, a mobile phase carries the sample through a stationary phase, and components distribute between phases according to their chemical affinity, yielding separation.
Common chromatography-based techniques include gas chromatography (GC), liquid chromatography (LC) such as high-performance liquid chromatography (HPLC)
Separation depends on factors: partition coefficients, adsorption, size, charge, and specific interactions with the stationary phase.
Applications span pharmaceutical analysis, proteomics, metabolomics, environmental monitoring, food and beverage safety, and chemical synthesis purification.
Typical chromatography systems combine a column packed with stationary phase, a controlled mobile-phase flow, and detectors
Considerations include method development time, cost of instruments and consumables, need for trained personnel, and potential
Chromatography originated with Mikhail Tsvet in the early 20th century; modern chromatography matured with developments in
As a generic term, chromatography-based methods are central to analytical chemistry and pharmaceutical development due to