cellefrie
Cellefrie refers to technologies and applications that operate without living cells. In biology, cell-free (cellefrie) systems use cell extracts, purified components, or reconstituted networks to perform biochemical reactions in vitro. The field grew from early cell-free protein synthesis experiments in the 1950s and 1960s and has expanded into practical platforms for research, development, and education.
Two main categories exist: lysate-based systems, which use crude extracts from bacteria, yeast, plants, or insect
Applications include rapid prototyping of proteins and pathways, production of proteins that are toxic to cells,
Advantages: speed, controllability, safety (no live organisms), and an open reaction environment that facilitates optimization. Limitations:
Recent trends involve shelf-stable, lyophilized formulations; integration with microfluidics and automation; and deployment for on-demand biomanufacturing