baseeditorit
Baseeditorit, or base editors, are engineered proteins that enable precise single-base substitutions in genomic DNA without introducing double-strand breaks. They combine a programmable DNA-targeting module, typically a Cas9 nickase or catalytically dead Cas9, with a DNA-modifying enzyme to convert one base within a defined editing window. The most common classes are cytidine base editors (CBEs) and adenine base editors (ABEs).
Mechanism and types: CBEs deaminate cytosine to uracil in the target DNA, which is read as thymine
History and development: The concept emerged with cytidine base editors such as BE3 and BE4 around 2016–2017,
Limitations and safety: Base editing is constrained by PAM availability, editing window size, and the potential
Applications: Base editors are used in functional genomics, disease modeling, and crop improvement. They enable precise