TrisGlycineSDS
TrisGlycineSDS, commonly referred to as Tris-Glycine-SDS buffer, is a running buffer used in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It provides the ionic mobility and denaturing conditions necessary for separating proteins primarily by their molecular weight. The presence of SDS denatures proteins and imparts a uniform negative charge, allowing separation to be size-dependent.
The standard composition of TrisGlycineSDS running buffer is 25 millimolar Tris base, 192 millimolar glycine, and
Preparation and use involve dissolving the salts in deionized water, adding SDS, and adjusting the volume and
Notes on variants: while 0.1% SDS is common, some protocols use slight deviations (for example, 0.05–0.2% SDS)