Tre2Bub2Cdc16
Tre2Bub2Cdc16 is a multicomponent regulatory complex described in eukaryotic cell-cycle control, named for its constituent proteins Tre2, Bub2, and Cdc16. The best-characterized aspect involves the Bub2-Cdc16 subcomplex, which forms a GTPase-activating protein (GAP) for Tem1, a small GTPase that drives the mitotic exit network (MEN) in budding yeast. By stimulating Tem1 GTP hydrolysis, Bub2-Cdc16 helps maintain Tem1 in an inactive state, delaying mitotic exit until the spindle is properly positioned.
Cdc16 is also a core subunit of the anaphase-promoting complex/cyclosome (APC/C), linking cell-cycle progression with ubiquitin-mediated
Localization studies indicate the complex associates with key mitotic structures such as spindle pole bodies and
In summary, Tre2Bub2Cdc16 is viewed as a regulatory module at the intersection of the MEN and APC/C