Singlestain
Singlestain is a term used in some scientific literatures to describe a staining approach that relies on a single dye to label a target within a biological sample. In this usage, a single reagent is designed to bind selectively to a molecule, structure, or class of biomolecules, allowing the target to be visualized without the use of multiple contrasting dyes.
Technique and interpretation: The assay typically involves applying one staining reagent to the specimen, followed by
Applications and scope: Singlestain has been discussed in contexts such as histology, microbiology, and cell biology
Limitations and considerations: While singlestain offers procedural simplicity and reduced reagent costs, it typically provides limited
Origin and usage: The term is not uniformly defined and may be used informally in different research