Sequenzierlauf

Sequenzierlauf, often translated as sequencing run or sequencing process, refers to the experimental procedure in molecular biology where a DNA or RNA sequencer is used to determine the order of nucleotide bases within a sample. This process is fundamental to various fields of biological research, including genomics, transcriptomics, and epigenomics. Modern sequencers employ different technologies, such as sequencing by synthesis, nanopore sequencing, or sequencing by ligation, each with its own advantages and applications.

A typical sequencing run begins with preparing the DNA or RNA sample, often involving fragmentation and library preparation. This library is then loaded onto the sequencing instrument. The instrument then performs a series of chemical and optical reactions, capturing data as the DNA or RNA strands are read. This raw data, often in the form of images or electrical signals, is then processed by sophisticated bioinformatics algorithms to generate a sequence of base calls (A, T, C, and G). The output of a sequencing run is typically a large dataset of these base calls, representing the genetic or transcriptomic information of the analyzed sample. The quality and accuracy of the sequencing run are crucial for downstream analyses.