Sangerbased

Sangerbased refers to a method or process that utilizes the Sanger sequencing technique as a foundational element. The Sanger sequencing method, also known as the chain-termination method, was developed by Frederick Sanger in 1977. It is a DNA sequencing technique used to determine the order of nucleotides within a DNA molecule. This method relies on the use of dideoxynucleotides, which are modified nucleotides that lack a hydroxyl group and thus terminate DNA strand elongation.

In a Sanger-based approach, DNA is amplified and then subjected to a sequencing reaction where it is copied by DNA polymerase in the presence of normal nucleotides and a small amount of fluorescently labeled dideoxynucleotides. Each dideoxynucleotide is labeled with a different color fluorescent dye, corresponding to one of the four bases (A, T, C, G). As the DNA polymerase synthesizes new strands, it randomly incorporates these dideoxynucleotides, causing the chain to terminate. The resulting DNA fragments, each ending with a specific labeled dideoxynucleotide, are then separated by size, typically using capillary electrophoresis. A laser excites the fluorescent dyes, and a detector records the color emitted by each fragment as it passes by. This sequence of colors directly corresponds to the nucleotide sequence of the original DNA.

Therefore, any application, research, or diagnostic tool that fundamentally relies on the principles and execution of Sanger sequencing can be described as Sangerbased. This could include historical DNA analysis, specific gene sequencing for genetic disorder diagnosis, or certain quality control measures in molecular biology where precise nucleotide identification is paramount. While newer sequencing technologies have emerged, Sanger sequencing remains relevant for certain applications due to its accuracy and reliability for shorter DNA fragments.