RNasin

RNasin is a trade name for a ribonuclease inhibitor protein. Ribonucleases (RNases) are enzymes that degrade RNA, making them a common nuisance in molecular biology laboratories where RNA integrity is crucial for experiments. RNasin acts by binding to RNases and thereby preventing them from degrading RNA molecules. This inhibition is non-covalent, meaning RNasin binds reversibly to the RNase. It typically functions by binding to the active site of the RNase, blocking its catalytic activity.

The primary use of RNasin is to protect RNA samples from degradation during various molecular biology procedures. This includes RNA extraction, RNA purification, reverse transcription, in vitro transcription, and RNA-based assays. By preventing RNA degradation, RNasin helps to ensure the integrity and yield of RNA, leading to more reliable experimental results. It is commonly added to buffers and solutions that come into contact with RNA.

RNasin is a protein of approximately 50 kDa in molecular weight. It is often purified from sources such as the placenta. The inhibitor works effectively against a broad range of RNases, making it a versatile tool for molecular biologists. Its inclusion in experimental protocols is a standard practice when working with RNA to preserve its structure and function.