Qcorr

Qcorr is a software tool designed for the analysis of single-molecule Förster Resonance Energy Transfer (smFRET) data. It is widely used in the field of biophysics and biochemistry to study the dynamics and interactions of biomolecules at the single-molecule level. The software is particularly noted for its ability to correct for spectral cross-talk and direct excitation, which are common issues in smFRET experiments.

Developed by researchers at the University of Zurich, Qcorr provides a user-friendly interface for processing and analyzing smFRET data. It supports various data formats and offers a range of features, including burst search, photon distribution analysis, and FRET efficiency calculation. The software also includes tools for visualizing data, such as histograms and time traces, which are essential for interpreting the results of smFRET experiments.

One of the key advantages of Qcorr is its ability to perform dual-channel analysis, which allows for the simultaneous analysis of donor and acceptor fluorescence. This feature is crucial for accurately determining FRET efficiencies and studying the conformational dynamics of biomolecules. Additionally, Qcorr includes algorithms for correcting for background noise and photon losses, which can significantly improve the quality of the data.

Qcorr is available as open-source software, making it accessible to researchers worldwide. It is compatible with various operating systems, including Windows, macOS, and Linux. The software is continuously updated and improved by its developers, ensuring that it remains a valuable tool for the scientific community.