MichaelisMentenmodellen
MichaelisMentenmodellen is a foundational mathematical framework in biochemistry for describing the rate of enzyme-catalyzed reactions with a single substrate under certain conditions. The rate equation expresses the reaction velocity v as a function of substrate concentration [S]: v = (Vmax [S])/(Km + [S]). Here Vmax is the maximal rate at saturating substrate and Km is the substrate concentration at which v is half of Vmax.
Vmax equals kcat [E]tot, where kcat is the turnover number (the number of substrate molecules converted per
Limitations include inapplicability to enzymes with multiple substrates, cooperative or allosteric effects, significant product inhibition, or
Applications of the model include estimating kinetic parameters (Km, Vmax) and catalytic efficiency (kcat/Km), comparing enzymes
Historically, the model is attributed to Leonor Michaelis and Maud Menten, introduced in the early 20th century