MiSeq
MiSeq is a benchtop next-generation sequencing instrument developed by Illumina, designed for small-scale sequencing projects, targeted panels, and microbial genomics. Introduced in 2011, the MiSeq uses Illumina's sequencing-by-synthesis (SBS) chemistry with reversible terminators. It combines a compact instrument body, integrated flow cell, optics, and fluidics for automated cycles of nucleotide incorporation on a flow cell, enabling parallel sequencing of many DNA fragments.
The platform supports paired-end sequencing with read lengths up to 2x300 bases using specific reagent kits;
Data produced are base-called reads which, after demultiplexing, are delivered as FASTQ files suitable for downstream
Applications include amplicon sequencing (such as 16S rRNA gene profiling), targeted gene panels, small genome sequencing,