Gelelektrofiltration
Gelelektrofiltration is a laboratory technique that combines a gel matrix with an applied electric field to separate and purify charged molecules. The method relies on electrophoretic migration within a gel, typically made of agarose or polyacrylamide, whose porous network acts as a molecular sieve while the electric field drives charged species through the matrix. Separation occurs according to a combination of size, shape and charge, allowing resolution of closely related species.
In practice, samples are prepared in buffer, loaded onto or into a gel or compatible filtration chamber,
Applications include analytical and preparative separation of nucleic acids and proteins, as well as certain small
Limitations include relatively low throughput and potential sample loss or damage from heat or denaturation, depending