GCaMP7f

GCaMP7f is a genetically encoded calcium indicator that belongs to the GCaMP family of fluorescent proteins. These indicators are widely used in neuroscience research to visualize and quantify changes in intracellular calcium concentration in living cells. GCaMP7f is a genetically encoded sensor, meaning its expression is driven by a promoter within the cell's DNA, allowing it to be produced within the cell itself.

The fluorescence of GCaMP7f is dependent on the binding of calcium ions. When calcium levels rise, the protein undergoes a conformational change that increases its fluorescence intensity. Conversely, when calcium levels decrease, the fluorescence returns to a lower baseline. This dynamic response allows researchers to track neuronal activity, which is often accompanied by transient changes in intracellular calcium.

GCaMP7f has been engineered for improved photostability and a faster response kinetics compared to earlier GCaMP variants. These characteristics make it suitable for recording rapid neuronal firing patterns and for long-term imaging experiments. Its spectral properties also allow for multiplexing with other fluorescent probes. GCaMP7f can be expressed in various cell types using viral vectors or transgenic animal models, providing a powerful tool for studying neural circuits and their function in both health and disease.