FLISA

FLISA may refer to different things, including a geographic location and a laboratory technique. Flisa is a village in Åsnes Municipality in Innlandet county, Norway.

Fluorescent immunosorbent assay, commonly abbreviated FLISA, is a type of immunoassay that uses fluorescent labels and fluorescence detection to quantify target analytes such as proteins, hormones, or pathogens. Like other immunoassays, it relies on specific antibody–antigen interactions, but detection is achieved through fluorescence rather than colorimetric readouts.

In a typical FLISA, the target molecule is immobilized on a solid surface or captured in solution. A primary antibody binds to the target, followed by a fluorescently labeled secondary antibody. After washing to remove unbound material, the fluorescence intensity is measured with a fluorometer or compatible reader. The signal is proportional to the amount of target present, enabling quantification. FLISA can offer higher sensitivity and a wider dynamic range than some colorimetric assays, and may allow multiplexing with different fluorophores.

Limitations include the need for appropriate fluorescence instrumentation, potential issues with photobleaching, and background autofluorescence from samples or plates. Proper controls and optimization are essential for accurate results. FLISA is used in research, clinical diagnostics, environmental monitoring, and food safety to detect and measure a variety of analytes.