Cullin2based
Cullin-2based refers to a subset of Cullin-RING ubiquitin ligases (CRLs) in which Cullin-2 serves as the scaffold for assembling the ubiquitin-proteasome degradation machinery. In these complexes, Cullin-2 coordinates with the RING finger protein RBX1, the Elongin B and Elongin C adaptors, and a substrate receptor that binds target proteins. The substrate receptor typically contains a motif that binds Elongin B/C, enabling recruitment to the Cul-2 scaffold; many receptors within this family are described as VHL-box containing proteins, with the classic example being the von Hippel-Lindau (VHL) tumor suppressor that specifies a CRL2^VHL complex.
Functionally, Cullin-2-based ligases act as E3 ubiquitin ligases that catalyze the transfer of ubiquitin from an
Regulation of CRL2 complexes mirrors other CRLs. Activity is controlled by neddylation of Cullin-2 with NEDD8,
Clinical and research relevance arises from CRL2’s roles in development, metabolism, and stress responses, and from