Home

CpH

CpH is an acronym used in several disciplines, and its exact meaning depends on the context. In computational chemistry and biophysics, CpH most often stands for constant pH, as in CpHMD (constant-pH molecular dynamics). In this approach, the protonation states of ionizable groups in a biomolecule are allowed to fluctuate during molecular dynamics simulations in response to the surrounding pH. Variants include discrete-state and continuous-titration-coordinate formulations. CpHMD enables estimation of pKa values, pH-dependent structural changes, and the effect of protonation on binding and catalysis. Typical outputs include protonation-state populations and pH-dependent free-energy profiles.

In acid-base and protein chemistry, CpH is sometimes used to denote the critical pH, the pH at

Because CpH is not a standardized term, it is essential to consult the source for its precise

which
a
species
undergoes
a
qualitative
change
in
protonation
state
or
charge,
affecting
properties
such
as
solubility,
crystallization,
or
activity.
Practically,
CpH
is
linked
to
pKa
values
via
titration
curves
and
Henderson-Hasselbalch
relationships;
it
is
determined
experimentally
by
titration
or
computationally
from
pKa
predictions.
meaning.
Related
concepts
include
pKa,
acid-base
titration,
and
the
methods
for
estimating
pH-dependent
behavior
in
macromolecules,
including
CpHMD.