Cas13based

Cas13based is a platform and associated technologies that leverage the CRISPR-Cas13 enzyme system for a variety of applications, primarily in molecular diagnostics and RNA manipulation. Cas13 is a family of RNA-guided RNases, meaning it can be programmed with a guide RNA to target and cleave specific RNA molecules. This programmability makes it a versatile tool for detecting and manipulating RNA.

The Cas13based system often refers to the development of diagnostic tests that utilize Cas13's ability to bind to and cut target RNA. When Cas13 finds its specific RNA target, it activates a "collateral cleavage" activity, meaning it can then non-specifically cleave other nearby RNA molecules. This collateral activity is typically coupled with a reporter molecule, such as a fluorescently labeled RNA probe. Upon target recognition and collateral cleavage, the reporter probe is cut, releasing a detectable signal. This enables highly sensitive and specific detection of RNA molecules, such as those from viruses or bacteria.

Beyond diagnostics, Cas13based technologies are explored for applications like gene editing (by targeting RNA transcripts for degradation or modification), RNA sequencing, and the development of novel therapeutics. The programmability and specificity of the Cas13 system are key advantages, offering potential for advancements in fields ranging from infectious disease detection to fundamental biological research. Development in this area focuses on improving detection sensitivity, multiplexing capabilities, and streamlining assay workflows for broader accessibility and application.