AlaGlySerVal

AlaGlySerVal, abbreviated AGSV, is a tetrapeptide consisting of the amino acids alanine, glycine, serine, and valine in that order. As a short peptide, AGSV serves as a simple model system in biochemistry and protein science. Its composition reflects a mix of hydrophobic and polar character: alanine and valine contribute hydrophobic side chains, glycine provides flexibility, and serine introduces a polar hydroxyl group that can participate in hydrogen bonding or undergo phosphorylation in larger proteins. Overall, the peptide is neutral in charge at physiological pH and has an approximate molecular weight of around 332 daltons.

In laboratory contexts, AGSV is used in peptide synthesis demonstrations, in studies of peptide conformation and dynamics, and as a standard or calibrant in mass spectrometry and HPLC methods because of its small size and well-defined sequence. It can be synthesized by standard solid-phase peptide synthesis and purified via high-performance liquid chromatography.

While AGSV can occur as a fragment within longer polypeptides, its properties are highly context-dependent when embedded in a larger structure. The sequence provides a minimal platform for exploring how sequence composition influences peptide behavior, such as folding tendencies, solubility, and interactions with solvents or other biomolecules.