ARF1dependent

ARF1dependent refers to cellular processes or phenomena that require the activity of ADP-ribosylation factor 1 (ARF1). ARF1 is a small GTP-binding protein that plays a crucial role in intracellular trafficking, particularly in the formation and budding of transport vesicles from the Golgi apparatus. Its activation is a key step in recruiting coat proteins, such as coat protein complex II (COPI), to Golgi membranes, which is essential for retrograde transport. Therefore, ARF1dependent processes are those that are initiated or modulated by the GTP-bound, active form of ARF1.

These processes include the assembly of COPI vesicles, which are involved in returning resident Golgi proteins to their correct compartments and retrieving escaped proteins from the endoplasmic reticulum. ARF1 also plays a role in the formation of other vesicular transport pathways, and its dysregulation has been linked to various cellular defects. Consequently, studying ARF1dependent mechanisms is vital for understanding fundamental aspects of protein sorting, membrane dynamics, and organelle homeostasis within eukaryotic cells. Research in this area often involves genetic manipulation of ARF1 or its regulators to observe the downstream effects on cellular function.