5GCTGGTGG3

5GCTGGTGG3 is described in literature as a short DNA oligonucleotide sequence. The notation indicates the sequence is oriented from the 5' end to the 3' end, with the nucleotides written as GCTGGTGG. The core sequence consists of eight nucleotides: G, C, T, G, G, T, G, G.

Composition and properties: The oligo is GC-rich, containing six guanine or cytosine bases out of eight, which gives a GC content of about 75%. High GC content can contribute to increased duplex stability and binding strength on a per-nucleotide basis, particularly for short sequences. The sequence’s length and composition influence its binding specificity, propensity to form secondary structures, and suitability for various assays.

Applications and considerations: In educational and theoretical contexts, 5GCTGGTGG3 is commonly used as a representative example of a short primer, probe, or binding sequence. In practical laboratory settings, its short length and high GC content can lead to non-specific binding or primer-dimer formation if used as a standalone primer. For functional use in PCR or hybridization assays, the oligo is typically integrated into a broader design that accounts for target specificity, annealing temperature, and potential modifications.

Genomic context and interpretation: As a standalone sequence, 5GCTGGTGG3 does not imply a specific gene or biological function. If used in databases or experiments, it is usually documented as an eight-nucleotide oligonucleotide with explicit orientation (5' to 3') and any intended experimental role (primer, probe, control, etc.).