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UFF is an acronym that can refer to several organizations and terms, but it most commonly denotes the Universidade Federal Fluminense, a public university in the state of Rio de Janeiro, Brazil.

The Universidade Federal Fluminense's main campus is in Niterói, with additional campuses across the state. It offers undergraduate and graduate programs through a network of faculties and institutes, spanning humanities, sciences, engineering, health, arts, and social sciences. Its origins lie in the mid-20th-century expansion of Brazil's federal university system, and it has grown into a major center for teaching, research, and community outreach. The institution emphasizes research production, technology transfer, and cultural engagement, and maintains partnerships with national agencies and international universities.

Governance is headed by a rector and governed by a university council, with academic units organized into faculties, institutes, and centers. The university supports student services, extension programs, and public-service activities that interact with local communities and regional development projects.

Beyond the Universidade Federal Fluminense, the acronym UFF is used by other organizations and terms in different languages and contexts; the intended meaning is determined by the surrounding material.