ribozymejen
Ribozymejen is a synthetic ribozyme designed as a programmable RNA catalyst capable of sequence-specific cleavage of RNA substrates. It illustrates how engineered RNA motifs can fold into an active site that promotes phosphodiester cleavage in the presence of divalent metal ions.
Structure: The molecule comprises a catalytic core with flanking substrate-binding arms. The core includes a unique
Mechanism: Catalysis proceeds via RNA-mediated acid-base catalysis with assistance from metal ions, typically Mg2+. The reaction
Substrates and specificity: Ribozymejen recognizes a defined RNA sequence embedded within a secondary structure, often requiring
Applications: In research contexts, ribozymejen is used as a model for programmable RNA cleavage and ligation.
Research status: The ribozymejen exists primarily in laboratory settings, with ongoing optimization for stability, target range,
See also: Ribozyme; Hammerhead ribozyme; Hairpin ribozyme; RNA catalysis.