ikkeRNasefrie

IkkeRNasefrie is a label indicating that a material, reagent, or environment is not certified as RNase-free. In laboratory contexts, RNase-free designations are used to prevent degradation of RNA. The prefix "ikke" signals the opposite: potential RNase contamination.

Ribonucleases (RNases) are enzymes that degrade RNA. They are ubiquitous, highly stable, and can persist on skin, clothing, surfaces, and equipment, making clean handling essential even after drying or washing.

In RNA-based workflows—such as RNA isolation, reverse transcription, qPCR, or RNA sequencing—contamination with RNases can compromise sample integrity and produce unreliable results. Consequently, items lacking RNase-free certification should be treated as potentially contaminated.

Labeling practices vary by region; some suppliers or labs mark items as "ikkeRNasefrie" to indicate a lack of RNase-free status. When such labeling is encountered, researchers should either decontaminate the item to RNase-free status or replace it with certified RNase-free equivalents.

Best practices for minimizing risk include using dedicated RNase-free consumables, maintaining clean zones, wearing gloves, decontaminating work surfaces with RNase-removing agents, using RNase-free water, and properly autoclaving or UV-treating equipment. Barrier pipette tips and certified sterile tubes further reduce exposure.

The term highlights the importance of quality control in RNA work. If the RNase status is uncertain, consult product documentation or supplier standards and treat materials accordingly to protect RNA integrity.