høyGC
høyGC is a term used to describe regions of DNA with high guanine–cytosine content, or GC-rich regions. GC content is the percentage of nucleotides that are guanine or cytosine in a sequence. Because G–C base pairs form three hydrogen bonds, GC-rich DNA is more thermally stable and has a higher melting temperature than AT-rich DNA.
GC content varies widely among organisms and across genomes. Bacterial GC content ranges roughly from 25% to
High GC content poses technical challenges for sequencing and amplification. GC-rich regions can exhibit poor coverage,
Analyses of høyGC commonly use sliding-window calculations of GC percentage, GC skew, and isochore or domain
In biology and medicine, høyGC content influences DNA stability, chromatin organization, and gene regulation. The concept