ddNTPresistant
ddNTPresistant refers to a phenomenon observed in certain molecular biology techniques, particularly DNA sequencing, where the normal process is impeded by the presence of dideoxynucleotide triphosphates (ddNTPs). ddNTPs are chain-terminating nucleotides that are crucial for Sanger sequencing. They lack a hydroxyl group at the 3' carbon, which prevents the formation of a phosphodiester bond with the next incoming nucleotide, thereby halting DNA synthesis.
In standard Sanger sequencing, a controlled amount of ddNTPs is added along with dNTPs. When a ddNTP
The term "ddNTPresistant" implies a situation where the incorporation of ddNTPs is less efficient than expected