citometrija

Citometrija is a technique used in flow cytometry, a method of analyzing individual cells based on their physical and chemical properties. The term "citometrija" is derived from the Greek words "kytos," meaning cell, and "metron," meaning measure. This technique allows for the simultaneous measurement of multiple parameters of a large number of cells, providing a comprehensive analysis of cellular characteristics.

Flow cytometry works by passing a single-cell suspension through a focused laser beam. As cells pass through the beam, they scatter light, and the scattered light is analyzed to determine the size and granularity of the cells. Additionally, fluorescent dyes or antibodies can be used to label specific cellular components, allowing for the detection and quantification of specific molecules or cell surface markers. The data collected is then analyzed using specialized software to generate histograms or other graphical representations, which can be used to identify and quantify different cell populations.

Citometrija is widely used in various fields of biology and medicine, including immunology, hematology, and oncology. It is particularly valuable for studying cell differentiation, cell cycle analysis, and the detection of rare cell populations. The technique's ability to analyze large numbers of cells quickly and accurately makes it an essential tool in modern biological research and clinical diagnostics.